Multiplex genomic DNA amplification for deletion detection
摘要
<p>The present invention relates to a method for detecting multiple DNA sequences simultaneously. The method involves amplification of multiple sequences simultaneously by annealing a plurality of paired oligonucleotide primers to single stranded DNA. One member of each pair is complimentary to the sense strand of a sequences and the other member is complimentary to a different segment of the anti-sense strand of the same sequence. The amplification occurs by alternately annealing and extending the primers. The invention also includes oligonucleotide primer sequences helpful in detecting genetic diseases and/or exogenous DNA sequences.</p>
申请公布号
EP0364255(B1)
申请公布日期
2001.05.23
申请号
EP19890310424
申请日期
1989.10.11
申请人
BAYLOR COLLEGE OF MEDICINE
发明人
CASKEY, CHARLES T.;CHAMBERLAIN, JEFFREY S.;GIBBS, RICHARD A.L.;RAINER, JOEL, E.;NGUYEN, PHI NGA