发明名称 |
Aequorin and obelin mutants with differing wavelengths and bioluminescence |
摘要 |
The invention relates to aequorin and obelin mutants whose emission is shifted with respect to wild type. The shift in emission is accomplished using a combination of mutations of amino acids within aequorin or obelin that affect bioluminescence; use of different types of chromophores, i.e., coelenterazines with variable emission characteristics; and modifications of the photoprotein with fluorophores that will allow for emission of light at longer wavelengths as a result of energy transfer. Additionally, an assay employing aequorin mutants to test for HIV-1 protease inhibitors is disclosed.
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申请公布号 |
US7345160(B2) |
申请公布日期 |
2008.03.18 |
申请号 |
US20040811138 |
申请日期 |
2004.03.29 |
申请人 |
UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION |
发明人 |
DAUNERT SYLVIA;DEO SAPNA KAMLAKAR;DIKICI EMRE;ROWE LAURA |
分类号 |
C07H21/04;C07K14/435;C12N9/06;C12P21/02;C12Q1/68;G01N33/53;G01N33/533 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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