EXPRESSION-CONTROLLING DNA

摘要

PURPOSE:To obtain the subject DNA contained in a 5' upper stream region from the transcription starting point of a waxy gene strongly expressed in the seed of a gramineous plant and capable of being bound to an extraneous gene to improve the ingredients of the seed or produce a substance in the seed. CONSTITUTION:A genomic DNA prepared from the leaf of rice by a conventional method is cleaved with a restriction enzyme, and subsequently ligated with lambda-phage to make a library. The library is subjected to a plaque hybridization using the waxy cDNA of the rice and a positive clone is screened. The positive clone is treated with a restriction enzyme to form reporter vectors successively deleted from the 5'-upper region. The presence or absence of the expressions of the reporter genes are measured to specify an expression- controlling region to obtain the objective expression-controlling DNA having a basic sequence of the formula, etc., contained in a 5'-upper flow region upper by -637 to 275 bases from the transcription-starting point of the rice waxy gene, and capable of being bound to an extraneous gene and introduced in a gramineous plant to improve the ingredients of the seed and produce a substance with the seed.