发明名称 |
Heterologous gene expression with poliovirus replicon |
摘要 |
The replication machinery of polio virus is used to express heterologous gene products, such as chloramphenicol acetyl transferase, in mammalian cells. Detectable expression following DNA transfection demonstrated that a polio replicon containing a foreign gene in the P1 region transcribed from an inducible promoter can be exported from the nucleus to the cytoplasm. The proteins in the P2/P3 region of the RNA can be translated and thereby render the RNA capable of replication. A stable cell line harbouring the polio replicon in the genome results in constitutive expression of chloramphenicol acetyl transferase or other heterologous gene product.
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申请公布号 |
US6156538(A) |
申请公布日期 |
2000.12.05 |
申请号 |
US19980145455 |
申请日期 |
1998.09.02 |
申请人 |
ANDREWS, DAVID W.;HUGHES, MARTIN J. G.;MURDIN, ANDREW D. |
发明人 |
ANDREWS, DAVID W.;HUGHES, MARTIN J. G.;MURDIN, ANDREW D. |
分类号 |
C07K14/105;C12N15/86;(IPC1-7):C12N15/63;C12N5/10;C12P21/00 |
主分类号 |
C07K14/105 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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