METHODS FOR REFOLDING PROTEINS CONTAINING FREE CYSTEINE RESIDUES
摘要
The present invention relates to novel methods for making and refolding insoluble or aggregated proteins having free cysteines in which a host cell expressing the protein is exposed to a cysteine blocking agent. The soluble, refolded proteins produced by the novel methods can then be modified to increase their effectiveness. Such modifications include attaching a PEG moiety to form PEGylated proteins.
申请公布号
WO0187925(A2)
申请公布日期
2001.11.22
申请号
WO2001US16088
申请日期
2001.05.16
申请人
BOLDER BIOTECHNOLOGY, INC.;ROSENDAHL, MARY, S.;COX, GEORGE, N.;DOHERTY, DANIEL, H.
发明人
ROSENDAHL, MARY, S.;COX, GEORGE, N.;DOHERTY, DANIEL, H.