DNA BINDING DOMAIN INTEGRATION VECTOR AND SET THEREOF, FUSION PROTEIN-CODING VECTOR AND SET THEREOF AND PRODUCTION METHOD THEREOF, DESTINATION VECTOR, PLANT CELL EXPRESSION VECTOR AND PRODUCTION METHOD THEREOF, KIT FOR PRODUCING PLANT CELL EXPRESSION VECTOR, TRANSGENIC METHOD, AND GENOME EDITING METHOD

摘要

PROBLEM TO BE SOLVED: To provide a method for efficiently introducing, into a plant cell, a plural of fusion proteins in which TALE DNA binding domain is linked to a protein having biological activities, such as a FokI DNA cleavage domain, and to provide a vector group usable for it.SOLUTION: The invention provides a DNA binding domain integration vector containing a first attL recombinant site, a TALE DNA binding domain insertion area, a gene coding a protein, a second attL recombinant site, in which the first attL recombinant site, the insertion area, the gene, and the second attL recombinant site are arranged from the upstream of a protein coding direction toward the downstream in this order, and no promoter is arranged between the first attL recombinant site and an insertion site. The invention also provides a destination vector that is recombinable with the DNA binding domain integration vector, and has a promoter pair facing the opposite direction; and a method and kit using those vectors.SELECTED DRAWING: Figure 1