Oligonucleotides possessing a primary amino group in the terminal nucleotide
摘要
The invention consists of compounds and methods for the synthesis of oligonucleotides which contain one or more free aliphatic amino groups attached to the sugar moieties of the nucleoside subunits. The synthetic method is versatile and general, permitting amino groups to be selectively placed at any position on oligonucleotides of any composition or length which is attainable by current DNA synthetic methods. Fluorescent dyes or other detectable moieties may be covalently attached to the amino groups to yield the corresponding modified oligonucleotide.
申请公布号
US5118800(A)
申请公布日期
1992.06.02
申请号
US19910661914
申请日期
1991.02.27
申请人
CALIFORNIA INSTITUTE OF TECHNOLOGY
发明人
SMITH, LLOYD M.;FUNG, STEVEN;KAISER, JR., ROBERT J.