Method of Gene Screening With Yeast Having Ergosterol Synthase Undergoing Inducible Expression

摘要

An expression of an ergosterol biosynthetic enzyme such as ERG6 and ERG3 is regulated with an inducible promoter, and transformation is performed in a state where the enzyme is expressed, and then screening or assay of drug-resistant colonies is performed in a state where the expression of the enzyme is repressed.