摘要 |
The present invention relates to a method for quantitative analysis of interactions between fluorescein-labeled HIF-1alpha (alpha) C-terminal peptides and cAMP-responsive element binding protein (CBP) or p300 proteins, and a method of screening inhibitors against the formation of HIF-1alpha-p300 or HIF-1alpha-CBP protein complexes using the above method. Therefore, the fluorescence polarization (FP) measurement method of the present invention is useful for systemically evaluating the HIF-1alpha-CBP or HIF-1alpha-p300 interactions, and examining the effects of posttranslational modifications (hydroxylation, S-nitrosylation, and phosphorylation) of the C-TAD peptide of HIF-1alpha on the recruitment of p300.
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