TECHNIQUE FOR MULTISTAGE DIFFERENTIAL CLONING AND CELL GROWTH CONTROL GENE

摘要

PROBLEM TO BE SOLVED: To provide a method for simply and surely cloning a gene specifically expressible in cells exhibiting specific physiological phenomena and a cell growth control gene identified by the method. SOLUTION: This method for multistage differential cloning is characterized by repeating operations to delete genes commonly expressible from a library of the genes respectively expressible in cells X and Y exhibiting different physiological phenomena three times or more and specifying 20-500 genes specifically expressible in the cell X. The cell growth control gene is specifically expressible in human cells in a resting stage of the cell period. In the gene, cDNA thereof has a specific base sequence.