Fast method for detection and/or identification of a single base in a nucleic acid sequence and its applications.
摘要
<p>The feature of this process is that: (1) the sequence on which the base to be identified is located is made hybrid with a nucleotide of adequate length; (2) the synthesis of the complementary strand of the hybrid obtained in (1) is started, said nucleotide acting as a trigger, in the presence of: a polymerase without 3'5' exonucleasis action; and at least one modified nucleotide base; (3) the incorporated blocking nucleotide base is detected by any suitable means. Application: diagnosis of genetic diseases.</p>
