Fluorescent detection method based on enzyme activated conversion of a fluorophore precursor substrate

摘要

Methods are provided for the fluorescent detection of an analyte of interest wherein an amine-substituted, ortho-fused pyrazine fluorophore, with neither nitrogen of the pyrazine ring being fused or substituted, is produced by enzymatic oxidation of a fluorophore precursor substrate which comprises a nitrogen-substituted, cyclic compound. Also provided are novel fluorophore-labelled compounds.