PROCESS FOR PREPARING HIGH-PURITY DERMATAN SULPHATE, AND PHARMACEUTICAL COMPOSITIONS WHICH CONTAIN IT

摘要

A process for preparing dermatan sulphate (DS) of pharmaceutical purity from animal organs rich in mucopolysaccharides (MPS), comprising the following essential stages: a) stabilising the fresh organs by freezing them either as such or in the form of powder, b) micronising the stable material containing the MPS with an aqueous CaC12 solution, c) digesting the homogenate comprising the raw material and the CaCl2 with proteolytic enzymes at alkaline pH and at low temperature, d) acidifying, heating and filtering the lysate, e) treating the filtrate with quaternary ammonium salts able to undergo complexing with and thus precipitate either the DS alone or all the MPS selectively, f) recovering and purifying the DS from the ammonium complex which contains it.