Gene transfer method with the use of serum-free medium

摘要

A method for transfening a gene into target cells by a retrovirus with the use of a serum-free medium. This method comprises infecting target cells with a retrovirus in a serum-free medium optionally containing low-density lipoprotein and/or cytokines in the presence of a functional substance such as fibronectin in an amount effective in elevating the gene transfer efficiency of the retrovirus into the target cells when the retrovirus and the target cells are present together.