发明名称 |
A method for immobilizing enzymes, and in particular, to a method for converting cell bound enzymes into cell mass particles. |
摘要 |
An improvement directed to the generation of particle form cell mass immobilized enzymes through cross-linking with polyazetidine prepolymer which comprises pretreating the enzymatically active microorganism cells in aqueous suspension with glutaraldehyde to effect partial cross-linking followed by dewatering and then admixing so-treated cell mass with the polyazetidine prepolymer followed by curing. …<??>The dispersion of treated enzymatically active cell mass with the polyazetidine prepolymer may be sub-divided and shaped into granular form prior to curing, so that the immobilized enzyme product comprises cell mass enzyme granules of desired size and a controlled shape in high yield. …<??>Glucose isomerase from a strain of the Streptomyces murinus cluster and tryptophan synthetase from a strain of E. coli are exemplified preferred embodiments. |
申请公布号 |
EP0206687(A1) |
申请公布日期 |
1986.12.30 |
申请号 |
EP19860304566 |
申请日期 |
1986.06.13 |
申请人 |
NOVO INDUSTRI A/S |
发明人 |
WUEMPELMANN, MOGENS |
分类号 |
C12N9/88;C12N11/08;C12P13/22;C12P19/24;C12R1/19;C12R1/465 |
主分类号 |
C12N9/88 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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