| 摘要 |
The present invention provides a method for detection of sequence variation of nucleic acid comprising steps of (a) preparing a nucleic acid which has a possibility of carrying sequence variation, (b) adding two or more different oligonucleotides, which are complementary to the nucleic acid, 5' end of which are different detectable marker-labeled and 3' end of which have sequence complementary to two or more different predetermined sequence variants of the nucleic acid respectively, (c) denaturing the nucleic acid by heating, (d) hybridizing the oligonucleotides to the nucleic acid and extending using thermostable DNA polymerase on the condition of decreasing the temperature gradually, and (e) detecting a extended nucleic acid product. The present invention provides simple and convenient method for sequence variations detection. |
