BIOMARKERS FOR DETECTION OF COLORECTAL CANCER

摘要

Provided are kits comprising biomarkers for detection of early stages (I and II) of colorectal cancer and methods for diagnosis and treatment of colorectal cancer at early stages (I and II).

主权项

1. A method for detecting cancer, comprising:
(a) analyzing a biological sample from a subject to detect a presence of a biomarker in the sample, comprising
i. obtaining a DNA sample from a subject;ii. digesting the DNA sample with a methylation-sensitive restriction enzyme in the presence of a glycol compound;iii. amplifying the digested sample of step (ii);iv. quantifying amplification results from step (iii) using a real-time quantitative PCR; andv. analyzing DNA methylation status within a recognition site of the methylation-sensitive restriction enzyme to detect a presence of the biomarker in the sample; (b) determining a methylation status of the biomarker detected in step (v); and (c) comparing the methylation status of the biomarker detected in the sample to cancer-positive and/or cancer-negative reference methylation status of the biomarker to detect whether the subject has cancer.