PROCESSES FOR DETECTING OR QUANTIFYING MORE THAN ONE NUCLEIC ACID IN A LIBRARY

摘要

This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention.

主权项

1. A process for detecting or quantifying more than one nucleic acid of interest in a library comprising the steps of:
a) providing
(i.) an array of fixed or immobilized nucleic acids complementary in part orwhole to sequences of said nucleic acids of interest;(ii.) a library of nucleic acid analytes which may contain the nucleic acids of interest sought to be detected or quantified;(iii.) polymerizing means for synthesizing nucleic acid copies of said nucleic acid analytes said polymerizing means comprising a first set of primers and a second set of primers;(iv.) means for addition of sequences to the 3′ end of nucleic acids; b) contacting said nucleic acid analytes with said first set of primers to form a first bound entity; c) extending said bound set of first set of primers by means of template sequences provided by said nucleic acid analytes to form first nucleic acid copies of said analytes; d) extending said first nucleic acid copies by the addition of non-template derived sequences to the 3′ end of said first nucleic acid copies; e) contacting said extended first nucleic acid copies with said second set of primers to form a second bound entity; f) extending said bound set of second set of primers by means of template sequences provided by said extended first nucleic acid copies to form second nucleic acid copies; g) separating said second nucleic acid copies from the said extended first nucleic acid copies; h) repeating steps e), f) and g) until a desirable amount of second nucleic acid copies have been synthesized; i) hybridizing said second nucleic acid copies formed in step h) to said array of nucleic acids provided in step (a)(i.); and j) detecting or quantifying any of said hybridized second nucleic acid copies obtained in step i).