发明名称 |
CYTOPLASMATISCHE EXPRESSION VON ANTIKÖRPERN, ANTIKÖRPERFRAGMENTEN UND ANTIKÖRPERFRAGMENT- FUSIONSMOLEKÜLEN IN E. COLI |
摘要 |
Prodn. of recombinant antibodies (Ab), Ab fragments or Ab fragment/enzyme fusion proteins, where the enzyme is a cytoplasmic mammalian or E. coli enzyme, is effected using a thioredoxinreductase deficient E. coli strain, the expression prods. being isolated from the cytoplasm. Also claimed are: (1) Ab fragment/enzyme fusion protein in which the enzyme is E. coli beta -glucuronidase; and (2) gene encoding the fusion protein of (1). |
申请公布号 |
AT242808(T) |
申请公布日期 |
2003.06.15 |
申请号 |
AT19960103913T |
申请日期 |
1996.03.13 |
申请人 |
HOECHST AKTIENGESELLSCHAFT |
发明人 |
OPPER, MARTIN, DR.;BOSSLET, KLAUS, DR.;CZECH, JOERG, DR. |
分类号 |
C12N15/09;A61K38/00;A61K39/395;C07H21/04;C07K16/00;C07K19/00;C12N9/24;C12N15/00;C12N15/13;C12N15/62;C12R1/19;(IPC1-7):C12N15/13 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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