BIOASSAY FOR DETECTING 2,3,7,8-TETRACHLORODIBENZO-PARA-DIOXIN AND TCDD-LIKE COMPOUNDS AND NOVEL RECOMBINANT CELL LINE USEFUL THEREFOR

摘要

A recombinant cell line deposited at the American Type Culture Collection under ATCC accession number CRL-12286, also known as the mouse H1L1.1 cell line, has been made by using genetic engineering techniques for inserting dioxin responsive elements upstream of a luciferase reporter gene, and then, transfecting the resultant recombinant expression plasmid, identified as pGudLuc1.1, into mouse hepatoma cells. The mouse H1L1.1 cell line is useful in a method for the quantitative analysis of polyaromatic hydrocarbons in samples.