发明名称 |
High-throughput diagnostic assay for the human virus causing severe acute respiratory syndrome (SARS) |
摘要 |
The present invention relates to a high-throughput diagnostic assay for the virus causing Severe Acute Respiratory Syndrome (SARS) in humans ("hSARS virus"). In particular, the invention relates to a high-throughput reverse transcription-PCR diagnostic test for SARS associated coronavirus (SARS-CoV). The present assay is a rapid, reliable assay which can be used for diagnosis and monitoring the spread of SARS and is based on the nucleotide sequences of the N (nucleocapsid)-gene of the hSARS virus. The present method eliminates false negative results and provides increased sensitivity for the assay. The invention also discloses the S (spike)-gene of the hSARS virus. The invention further relates to the deduced amino acid sequences of the N-gene and S-gene products of the hSARS virus and to the use of the N-gene and S-gene products in diagnostic methods. The invention further encompasses diagnostic assays and kits comprising antibodies generated against the N-gene or S-gene product.
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申请公布号 |
US7547512(B2) |
申请公布日期 |
2009.06.16 |
申请号 |
US20040807807 |
申请日期 |
2004.03.24 |
申请人 |
THE UNIVERSITY OF HONG KONG |
发明人 |
PEIRIS JOSEPH S. M.;YUEN KWOK YUNG;POON LIT MAN;GUAN YI;CHAN KWOK HUNG;NICHOLLS JOHN M.;LEUNG FREDERICK C. |
分类号 |
C12Q1/68;A61P11/00;A61P31/14;C12N15/50;C12Q1/00;C12Q1/70 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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