Substantially pure mammalian proteins having the following characteristics are disclosed: (a) molecular weight of approximately 110,000 Daltons; (b) bind HDL3; (c) bind apoA-I proteoliposomes and apoA-II proteoliposomes; and (d) contain approximately 10 % carbohydrate by weight. The present invention also discloses a substantially pure protein having the following characteristics: (a) a molecular weight of approximately 38,000 Daltons; (b) binds HDL3; and (c) contains approximately 331 amino acid residues. DNA sequences encoding these proteins, as well as cells transfected or transformed with an expression vector containing such a DNA sequence are also disclosed. Antibodies that bind to these proteins, methods for isolating an HDL3-binding protein and methods for detecting a genetic defect in an HDL-binding protein in a patient are also disclosed.
申请公布号
WO9005744(A1)
申请公布日期
1990.05.31
申请号
WO1989US05169
申请日期
1989.11.17
申请人
THE BOARD OF REGENTS OF THE UNIVERSITY OF WASHINGT;ZYMOGENETICS, INC.
发明人
ORAM, JOHN, F.;MCKNIGHT, GARY, L.;HART, CHARLES, E.;CURTIS, DEE, ANN