| 摘要 |
PROBLEM TO BE SOLVED: To provide a method by which the base sequence of a fundamentally single nucleic acid molecule can highly accurately and directly be determined without amplifying the chain of the nucleic acid. SOLUTION: The method for determining the base sequence of a single strand nucleic acid molecule of analysis target immobilized on a substrate with a scanning probe microscope is characterized by having (I) a process for analyzing the shape of bases along the nucleic acid chain of the single strand nucleic acid molecule with a probe, (II) a process for analyzing the presence or absence of the interaction of each base with a standard nucleic acid along the nucleic acid chain of the single strand nucleic acid molecule with a probe in which the standard nucleic acid is formed, and (III) a process for specifying the kinds of the existing bases and determining the base sequence of the nucleic acid chain, on the basis of the results obtained by the two processes. COPYRIGHT: (C)2007,JPO&INPIT
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