Method of detecting thalassemia by optical analysis of blood components

摘要

The method of detecting thalassemia by optical analysis of blood components is a spectral detection method that is based on the fluorescence spectra of a set of biomolecules, including tyrosine, tryptophan, nicotinamide adenine dinucleotide, and flavin adenine dinucleotide, which are all found in blood plasma, and porphyrin, which is found in red blood cells (RBCs). Measured ratios of intensity maxima between tryptophan and nicotinamide adenine dinucleotide, flavin adenine dinucleotide and nicotinamide adenine dinucleotide, tyrosine and tryptophan, and the normal form of porphyrin and the basic form of porphyrin may each be used, alone or in combination, to diagnose a patient as suffering from thalassemia.

主权项

1. A method of detecting thalassemia by optical analysis of blood components, comprising the steps of:
performing synchronous fluorescence excitation spectroscopy on a blood plasma sample from a patient; identifying and measuring fluorescence intensity maxima in an excitation band corresponding to tryptophan and nicotinamide adenine dinucleotide; defining an intensity ratio as a ratio of measured fluorescence intensity of tryptophan to measured fluorescence intensity of nicotinamide adenine dinucleotide; and diagnosing the patient as suffering from thalassemia if the intensity ratio is about 6.2.