| 摘要 |
FIELD: biotechnology, biochemistry, microbiology. SUBSTANCE: method involves cells disruption by ultrasonic oscillation, thermal treatment at 58-60 C for 5-10 min, not less, centrifugation, chromatography on DEAE-cellulose (DE-52), an additional purification on phosphocellulose P-11, heparin-Sepharose and the concentration against 50% glycerol solution. The yield of enzyme is 170 000-200 000 U/10 g of wet biomass. Method ensures to obtain the enzyme no containing impurities of nonspecific nucleases and phosphatases and at the low level of protein. EFFECT: improved method of producing. 4 cl, 1 tbl, 2 dwg, 4 ex |
