Methods and compositions for exosome isolation

摘要

Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.

主权项

1. A method for the isolation of exosomes from a biological fluid sample comprising:
a) adding polyethylene glycol having a molecular weight of from 3000 to 8000 daltons to the biological fluid sample, b) incubating the biological fluid sample with the polyethylene glycol, c) isolating the precipitated exosomes from the biological fluid sample; and d) isolating nucleic acid from the exosomes.