发明名称 |
Verfahren zur Bestimmung von Dicyandiamid in Pflanzen oder Pflanzenteilen |
摘要 |
The present invention is a method for determination of dicyanodiamide in a plant matrix, i.e. in a plant or a part of a plant. It involves a series of steps including extracting any dicyanodiamide from the plant matrix as well as purifying the dicyanodiamide obtained of interfering substances. Next, the dicyanodiamide obtained is reacted with 3-hydroxy-2-butanone in a non-aqueous, alcoholic hydrochloric acid medium to form 2-guanidino-4,5-dimethyloxazole, and then any 2-guanidino-4,5-dimthyloxazole formed is isolated using liquid-liquid extraction. Finally, any isolated 2-guanidino-4,5-dimethyloxazole is purified by reverse phase high pressure liquid chromatography (HPLC) and the amount of 2-guanidino-4,5-dimethyloxazole is determined spectrophotometrically at a wavelength of 260 nm. |
申请公布号 |
DE3738675(C1) |
申请公布日期 |
1988.12.08 |
申请号 |
DE19873738675 |
申请日期 |
1987.11.13 |
申请人 |
SKW TROSTBERG AG, 8223 TROSTBERG, DE |
发明人 |
RUST, ULRICH, DR., 8223 TROSTBERG, DE |
分类号 |
G01N33/52;(IPC1-7):G01N33/50;G01N30/02 |
主分类号 |
G01N33/52 |
代理机构 |
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