| 摘要 |
<p>New methods are disclosed for detecting cancer associated genes, and obtaining corresponding cDNA sequences. The methods involve displaying cDNA from control cells, and from a plurality of different cancer cells that share a duplicated or deleted gene in the same region of a chromosome. Four novel genes associated with cancer have been identified. In at least about 60% of the breast cancer cell lines tested, RNA hybridizing with the cDNAs were substantially more abundant than in normal cells. Many of the cell lines also showed a duplication of the corresponding gene, which probably contributed to the increased level of RNA in the cell. However, for each of the four genes, there were some cell lines which had RNA overabundance without gene duplication. This suggests that the gene product is sufficiently important to the cancer process that cells will use several alternative mechanisms to achieve increased expression. One of the protein products is predicted to be a membrane protein, another is predicted to have DNA or RNA binding activity. Several of the genes share conserved regions with distantly related organisms, suggesting a fundamental role in cellular metabolism or proliferation.</p> |
