Lateral flow assays for tagged analytes

摘要

Methods of determining whether an analyte is present in a sample using a competitive assay are provided. Aspects of the methods include employing a competitor that provides for the obtainment of signal which is directly proportional to the amount of analyte in the sample. Also provided are devices and kits that find use in practicing the methods described herein.

主权项

1. A method of determining whether an analyte is present in a sample, the method comprising:
(a) providing a sample-contacted sample receiving region of a test lateral flow assay device, wherein the sample-contacted sample receiving region comprises:
(i) a quantity of the sample;(ii) an immobilized first capture probe that specifically binds to the analyte; and(iii) a competitor comprising a first binding pair member that specifically binds to the first capture probe; and (b) reading a detection region of the test lateral flow assay device to determine whether the analyte is present in the sample, wherein the detection region comprises an immobilized detection capture probe that specifically binds to the competitor.