| 摘要 |
Disclosed are mammalian tau proteases, as well as proteolytically-active fragments, variants, and mutants thereof. Also disclosed are polynucleotides and recombinant expression vectors that encode these polypeptides, as well as methods for producing such proteins in selected recombinant host cells, and for using the compositions in a variety of diagnostic and analytical assays. |
| 主权项 |
1. A method of identifying a serine protease inhibitor, the method comprising contacting a compound suspected of inhibiting serine protease activity with a peptide or polypeptide comprising enhanced or diminished serine protease activity when compared to that of a naturally occurring peptide or a naturally occurring polypeptide, and an amino acid sequence that is (i) at least 95%, (ii) at least 96%, (iii) at least 97%, (iv) at least 98%, or (v) at least 99% identical to (A) an at least 50-amino acid contiguous sequence from SEQ ID NO:6, (B) an at least 60-amino acid contiguous sequence from SEQ ID NO:6; (C) an at least 70-amino acid contiguous sequence from SEQ ID NO:6; (D) an at least 80-amino acid contiguous sequence from SEQ ID NO:6; or (E) an at least 90-amino acid contiguous sequence from SEQ ID NO:6 and wherein the peptide or polypeptide comprises at least one substitution at a position that corresponds to amino acid residue 5, 257, 260, 266, 272, 273, 279, 280, 296, 301, 303, 304, 315, 317, 332, 335, 336, 337, 342, 363, 369, 389, 406 or 427 of SEQ ID NO:6, wherein inhibition of the tau protease activity by the compound is indicative of serine protease inhibitory activity of the compound. |
