| 摘要 |
PURPOSE:To enable highly accurate measurement of protein in urine, by adding a chelate agent allowed to bond to molybdenum into a measuring reagent mainly composed of pigment and molybdenum or by adding metal ion allowed to bond to oxalic acid, citric acid and phosphor and salts thereof expected coexist in a sample but failing to react with the pigment beforehand to avoid phenomenon indicating a negative value by normal urine. CONSTITUTION:Pigment such as pyrogallol red and pyrocatechol violet forms a complex with molybdenum, which shifts the wavelength to the long wavelength side as protein. When a colorimetric assay of protein, especially urinous protein utilizing this, a chelate agent existing in a sample, potentially causing a negative value, namely, organic acid or/and phosphates or a chelate agent having the same action as these is added to a reagent beforehand or metal ion allowed to bond to oxalic acid, citric acid, phosphoric acid and salts thereof expected to coexist in the sample, but failing to react with said pigment is added thereto. The chelate therein used is ethylene diamine tetraacetate (EDTA), tripolyphosphoric acid, metaphosphoric acid or the like. The addition thereof shall be normally in a range of 0.001-1%. |
