Homogeneous method for assay of double-stranded nucleic acids using fluorescent dyes and kit useful therein

摘要

A highly sensitive homogeneous assay allows for quantitative detection of amplified nucleic acids. This detection is achieved during or after amplification with a high affinity fluorescent dye which is from the class of unsymmetrical cyanine dyes having at least two positive charges and a binding constant (Kb) within the range of from about 1x104 to about 5x105 (molar-1). The reagents used for the assay can be contained in a kit designed for amplification such as by polymerase chain reaction.