发明名称 IN-SITU CELL EXTRACTION AND ASSAY METHOD
摘要 A method of assaying for an analyte comprises the steps of: i) mixing a sample of cells possibly containing the analyte with a cell lysis reagent to provide a cell lysis fluid, ii) mixing the cell lysis fluid with reagents, including a specific binding partner of the analyte for binding to the analyte, for performing a specific binding assay for the analyte, iii) and mixing the cell lysis fluid with a sequestrant for the cell lysis reagent, whereby the binding of step ii) is performed in the presence of the sequestrant.
申请公布号 CA2230423(A1) 申请公布日期 1998.09.03
申请号 CA19982230423 申请日期 1998.02.24
申请人 AMERSHAM PHARMACIA BIOTECH UK LIMITED 发明人 HORTON, JEFFREY KENNETH
分类号 G01N33/531;G01N33/68;G01N33/84;G01N33/88;(IPC1-7):G01N33/53;G01N1/38;C12Q1/68 主分类号 G01N33/531
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