发明名称 |
METHOD OF MEASURING CANCER RELATED SUBSTANCES BY RAMAN SPECTROSCOPY |
摘要 |
A method for measuring cancer related substances including cancer cell-derived free DNA by Raman spectroscopy, involving steps for preparing a biochip having a meso-crystal region of silver oxides containing a silver peroxide, adding a blood serum or a biological sample solution dropwise onto the meso-crystal region of said biochip, selectively trapping the cancer-related substances having a positive charge in the sample, irradiating the trapped cancer-related substance with an exciting laser light and detecting a surface enhanced Raman scattering therefrom, wherein cancer diseases are evaluated on the basis of the intensity of the Surface Enhance Raman Scattering (SERS). In the carbon-specific D band and G band in the Raman scattering spectrum, a characteristic peak spectrum of the cancer-related substance can be detected in the proximity of the methyl group characteristic of 2900 cm−1. |
申请公布号 |
US2016187344(A1) |
申请公布日期 |
2016.06.30 |
申请号 |
US201514933213 |
申请日期 |
2015.11.05 |
申请人 |
Ito Hiroaki;MYTECH CO., LTD. |
发明人 |
Ito Hiroaki;Hasegawa Yuki;Hasegawa Katsuyuki |
分类号 |
G01N33/574;G01N21/65 |
主分类号 |
G01N33/574 |
代理机构 |
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代理人 |
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主权项 |
1. A method of measuring a quantity of cancer related substances by Surface Enhanced Raman Scattering (SERS), the method comprising the steps of preparing a biochip having a meso-crystal region of silver oxides containing a silver peroxide, adding dropwise a serum or biological liquid sample to the meso-crystal region of the biochip, selectively trapping cancer related substance having a positive charge in the sample, irradiating the trapped cancer related substance with an exciting light, and detecting the surface enhanced Raman scattering light. |
地址 |
Tokyo JP |