发明名称 |
Compositions and methods for protein structural determinations |
摘要 |
A method for determining three-dimensional structural information of a protein involves producing the protein in a form substantially labeled with <13>C or <15>N or both or substantially labeled with <15>N and <13>C and partially labeled with <2>H and subjecting the protein to nuclear magnetic resonance spectroscopic analysis. The isotopically labeled protein is produced by a method which involves producing a substantially labeled microbial protein hydrolysate, subjecting the protein hydrolysate to cation exchange chromatography to produce a partially purified labeled amino acid mixture, subjecting the partially purified labeled amino acid mixture to anion exchange chromatography to produce a purified labeled amino acid mixture and supplementing the purified labeled amino acid mixture with isotopically labeled cysteine and optionally with isotopically labeled glutamine and asparagine. |
申请公布号 |
AU3608797(A) |
申请公布日期 |
1997.12.04 |
申请号 |
AU19970036087 |
申请日期 |
1997.08.28 |
申请人 |
MARTEK BIOSCIENCES CORPORATION |
发明人 |
JONATHAN MILES BROWN |
分类号 |
C12N5/06;B82B3/00;C07B59/00;C07C229/24;C07C231/12;C07C237/06;C07C323/58;C12N1/12;C12P13/04;C12P21/02;C12Q1/02;C12Q1/04;G01N24/08;G01N30/62;G01N30/88;G01N33/483;G01N33/534;G01N33/58;G01N33/60;G01N33/68;G01R33/465;(IPC1-7):G01N24/08 |
主分类号 |
C12N5/06 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|