摘要 |
The current invention provides a method of calculating the volume of a sample mixed into a known volume of an assay reaction mixture wherein the assay reaction mixture comprises a solute at a known concentration, wherein the solute is non-reactive with other constituents of the assay reaction mixture, is non-reactive with the constituents of the sample, is absent in the sample, and does not participate in the assay reaction. The current invention also pertains to assay reaction mixtures used in the methods of the current invention. |