| 摘要 |
<p>PCT No. PCT/SE97/00763 Sec. 371 Date Jul. 18, 1997 Sec. 102(e) Date Jul. 18, 1997 PCT Filed May 7, 1997 PCT Pub. No. WO98/03666 PCT Pub. Date Jan. 29, 1998The present invention relates to recombinant vectors which are useful for direct selection of colonies harboring recombinant plasmids, on basis of the loss of expression of a gene coding for an AsiA polypeptide from E. coli bacteriophage T4, the expression of which would normally have resulted in toxicity to the bacterial host. The invention also relates to host cells harboring the vectors, as well as to methods utilizing the vectors, for the selection of nucleic acid clones.</p> |
