UNIVERSAL SOLID SUPPORTS AND METHODS FOR THEIR USE

摘要

Universal solid support oligonucleotide synthesis reagents, oligonucleotide synthesis processes, and reagents for cleaving oligonucleotides from solid supports are disclosed. Oligonucleotide synthesis reagents have the following general formula:SS-R6-O-R3Iwherein SS is a solid support; R6 is where R5 is hydrogen or alkyl and R4 is a phosphate protecting group; and R3 is a ring moiety having vicinal groups -XR1 and -YR2 wherein each of X and Y is independently selected from the group consisting of O, S and NH and one of R1 and R2 is a blocking moiety and the other is hydrogen or a hydroxy protecting group. Oligonucleotide cleaving reagents include methylamine and/or ammonium hydroxide and trimethylamine.