发明名称 |
METHOD OF PREPARING A RADIOACTIVE RHENIUM COMPLEX SOLUTION. |
摘要 |
<p>The present invention provides methods and reagents for the estimation of the quantity of human DNA contained in a sample. Immobilized sample DNA is hybridized to a biotinylated oligonucleotide probe that hybridizes to a human genomic or mitochondria DNA sequence. The subsequent binding of streptavidin-horseradish peroxidase to the bound probe allows for chemiluminescent detection using a luminol-based reagent and X-ray film. In addition, the present invention provides methods and reagents to assess the quality of DNA contained in a sample. The sample is first size fractionated by agarose gel electrophoresis, and then immobilized, hybridized to a biotinylated oligonucleotide probe, and detected using the chemiluminescent method as used in the quantity estimation methods of the present invention.</p> |
申请公布号 |
GR3029875(T3) |
申请公布日期 |
1999.07.30 |
申请号 |
GR19990400964T |
申请日期 |
1999.04.05 |
申请人 |
F. HOFFMANN-LA ROCHE AG |
发明人 |
REYNOLDS, REBECCA L.;WALSH, P. SEAN |
分类号 |
C12N15/09;C12Q1/68;G01N33/53;(IPC1-7):C12Q1/68;C07H21/04;C12P19/34 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|