Detection of conductive polymer-labeled analytes

摘要

The disclosure relates to the detection of analytes (e.g., biological pathogens such as bacteria or viruses) using a conductive polymer label. The disclosed detection system utilizing the conductive polymer label generally involves the formation of an analyte conjugate between the target analyte and a conductive polymer moiety conjugated to the target analyte. The conductive polymer portion of the analyte conjugate is electrically activated to form an electrically activated analyte conjugate having an increased electrical conductivity relative to the analyte conjugate as originally formed. The electrically activated analyte conjugate can then be detected by any suitable means, such as by conductimetric or electrochemical detection.

主权项

1. A system for binding a target analyte, the system comprising:
(a) a magnetic nanoparticle capture composition comprising: (i) a magnetic nanoparticle; and (ii) a first binding pair member bound to the magnetic nanoparticle, the first binding pair member being capable of specifically binding to the target analyte; and (b) a conductive polymer nanoparticle label composition comprising: (i) a conductive polymer nanoparticle, and (ii) a second binding pair member bound to the conductive polymer nanoparticle, the second binding pair member being capable of specifically binding to the target analyte.