发明名称 |
Method of analysis and manipulation of DNA utilizing mismatch repair systems |
摘要 |
A diagnostic method for detecting a base pair mismatch in a DNA duplex, comprising the steps of contacting at least one strand of a first DNA molecule with the complementary strand of a second DNA molecule under conditions such that base pairing occurs contacting a DNA duplex potentially containing a base pair mismatch with a mispair recognition protein under conditions suitable for the protein to form a specific complex only with the DNA duplex having a base pair mismatch, and not with a DNA duplex lacking a base pair mismatch, and detecting any complex as a measure of the presence of a base pair mismatch in the DNA duplex.
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申请公布号 |
US6008031(A) |
申请公布日期 |
1999.12.28 |
申请号 |
US19970896518 |
申请日期 |
1997.06.17 |
申请人 |
DUKE UNIVERSITY |
发明人 |
MODRICH, PAUL L.;SU, SHIN-SAN;AU, KARIN G.;LAHUE, ROBERT S.;COOPER, DEANI LEE;WORTH, JR., LEROY |
分类号 |
C12N9/24;C12Q1/68;(IPC1-7):C12N9/24 |
主分类号 |
C12N9/24 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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