发明名称 |
Precursor miRNA loop-modulated target regulation |
摘要 |
Modulation of mRNA activity is achieved with precursor miRNAs (ta-RNAs). ta-RNAs, primarily pre-miRNAs and pri-miRNAs, including truncated and mutated ta-RNAs, are employed for modulation of mRNA expression where it is found that pri- and pre-miRNA have activity independently of the presence of functional mature miRNAs. Modification of at least one of the stem and loop of the ta-RNAs to enhance binding of the ta-RNA to the target mRNA is employed. The modification may be enhanced complementarity between the ta-RNA and the target mRNA and/or improved thermodynamic efficiency in binding of the ta-RNA to the target. |
申请公布号 |
US9388466(B2) |
申请公布日期 |
2016.07.12 |
申请号 |
US201414457656 |
申请日期 |
2014.08.12 |
申请人 |
Board of Regents of the Leland Stanford Jr Univ |
发明人 |
Chen Chang-Zheng;Trujillo Robin;Yue Sibiao |
分类号 |
C12Q1/68;C12N15/11;C12N5/0783;C07H21/04;A61K48/00 |
主分类号 |
C12Q1/68 |
代理机构 |
Casimir Jones, S.C. |
代理人 |
Casimir Jones, S.C. ;Bond Jason |
主权项 |
1. A method for identifying the role of a loop sequence of a precursor miRNA among a plurality of isoforms differing in their loop sequences, said method comprising:
substituting one loop from one isoform with an equivalent loop from a different isoform to produce a mutated precursor miRNA; introducing said one isoform in a first cell and said mutated precursor miRNA into a second cell; determining the effect of the one isoform and said mutated precursor miRNA on the phenotype of each of said first and second cells; wherein said phenotype is different, indicating that said loop sequence affects the role of said precursor miRNA; and identifying said loop sequence as affecting the role of said precursor miRNA. |
地址 |
Palo Alto CA US |