发明名称 |
Method of sequencing nucleic acid colonies formed on a surface by re-seeding |
摘要 |
A method of sequencing nucleic acids, which can include steps of (a) providing a substrate having a surface having a repeating pattern of features, wherein the features are spatially separated from each other on the surface of the substrate; (b) contacting the repeating pattern of features with a solution of different target nucleic acids to seed a subset of the features that contact the solution, wherein no more than the subset of the features that contact the solution is seeded with the target nucleic acids; (c) amplifying the target nucleic acids at the subset of features; (d) repeating steps (b) and (c) to increase the number of features that are seeded with a nucleic acid, thereby making an array of different nucleic acid colonies; and (e) detecting sequencing reactions at the different nucleic acid colonies on the surface. |
申请公布号 |
US9416415(B2) |
申请公布日期 |
2016.08.16 |
申请号 |
US201514641054 |
申请日期 |
2015.03.06 |
申请人 |
Illumina, Inc. |
发明人 |
Ronaghi Mostafa;Eltoukhy Helmy A. |
分类号 |
C12P19/34;C12Q1/68 |
主分类号 |
C12P19/34 |
代理机构 |
Illumina, Inc. |
代理人 |
Murphy John T.;Illumina, Inc. |
主权项 |
1. A method of sequencing nucleic acids, comprising
(a) providing a substrate comprising a surface having a repeating pattern of features, wherein the features are spatially separated from each other on the surface of the substrate; (b) contacting the repeating pattern of features with a solution of different target nucleic acids to seed a subset of the features that contact the solution, wherein no more than the subset of the features that contact the solution is seeded with the target nucleic acids; (c) amplifying the target nucleic acids at the subset of features; (d) repeating steps (b) and (c) to increase the number of features that are seeded with a nucleic acid, thereby making an array of different nucleic acid colonies; and (e) detecting sequencing reactions at the different nucleic acid colonies on the surface. |
地址 |
San Diego CA US |