发明名称 |
MODIFIED NUCLEOSIDES, NUCLEOTIDES, AND NUCLEIC ACIDS, AND USES THEREOF |
摘要 |
The present disclosure provides modified nucleosides, nucleotides, and nucleic acids, and methods of using thereof. |
申请公布号 |
US2016264975(A1) |
申请公布日期 |
2016.09.15 |
申请号 |
US201615143364 |
申请日期 |
2016.04.29 |
申请人 |
Moderna Therapeutics, Inc. |
发明人 |
Schrum Jason P.;Siddiqi Suhaib;Ejebe Kenechi |
分类号 |
C12N15/113 |
主分类号 |
C12N15/113 |
代理机构 |
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代理人 |
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主权项 |
1. A method of synthesizing a messenger ribonucleic acid (mRNA), comprising the steps of:
a) providing a complementary deoxyribonucleic acid (cDNA) that encodes a pharmaceutical protein of interest; b) selecting a nucleotide that disrupts a binding of a major groove binding partner with the RNA, wherein the nucleotide has decreased binding affinity to the major groove binding partner selected from the group consisting of toll-like receptor (TLR) 3, TLR7, TLR8, retinoic acid-inducible gene I (RIG-I), melanoma differentiation-associated gene 5 (MDA5) and laboratory of genetics and physiology 2 (LGP2), and wherein the nucleotide comprises a modification on the major groove face of the nucleobase where an atom of the major groove face of the nucleobase is replaced or substituted with an alkyl group; c) contacting the provided cDNA and the selected nucleotide with an RNA polymerase under conditions such that an RNA transcript is synthesized, and d) 5′-capping the RNA transcript concomitantly or post-transcriptionally such that the mRNA is synthesized. |
地址 |
Cambridge MA US |