Trail mutant membrane-penetrating peptide-alike and methods of preparation thereof

摘要

The invention mainly relates to the field of genetic engineering drugs, in particular to a mutant cDNA sequence obtained by mutating valine at position 114, glutamate at position 116, glycine at position 118, proline at position 119 and glutamine at position 120 in an amino acid sequence at positions 114-281 of an extracellular fragment of a wild-type TRAIL protein respectively into arginines, so as to allow amino acids at positions 114-121 of the TRAIL protein to form a 8-consecutive arginine sequence, and then by gene synthesis and PCR mutation and splicing; and the TRAIL mutant has excellent therapeutic effect for a variety of tumors of different types, and is a new generation of promising drug for highly efficiently inducing tumor cell apoptosis.

主权项

1. A TRAIL Mutant Membrane-Penetrating Peptide-alike,
wherein the mutant is a protein comprising a Membrane-Penetrating Peptide-alike structure formed by selectively changing an amino acid coding sequence at positions 114-121 of an extracellular fragment of a TRAIL wild-type protein as shown in SEQ ID NO: 5 to a TRAIL mutant protein as shown in SEQ ID NO: 2, namely mutating valine at position 114 into arginine, glutamate at position 116 into arginine, glycine at position 118 into arginine, proline at position 119 into arginine and glutamine at position 120 into arginine, so as to allow the N-terminal of the mutant protein to form an 8-consecutive arginine coding sequence.