Method for multiplex-detecting chronic myelogenous leukemia gene using cleavable probe

摘要

One embodiment of the present invention provides a detection kit for detecting a chronic myelogenous leukemia (CML) gene expression, wherein the detection kit includes a primer set which is specifically bound to the CML gene; and a cleavable probe which is specifically bound to the inside of a CML gene amplification product which is amplified by the primer set. Another embodiment of the present invention provides a method of measuring the CML gene expression by using the detection kit according to one embodiment of the present invention. The method according to one embodiment of the present invention is used to efficiently detect a low CML gene expression for CML diagnosis and prognosis diagnosis.

主权项

1. A method of detecting an e13a2, e14a2, e13a3, or e14a3 breakpoint of a CML gene, the method comprising:
obtaining DNA from a sample; mixing at least one primer set, a cleavable probe of SEQ ID NO: 33, and the obtained DNA sample to prepare a mixed sample, wherein the at least one primer set is selected from the group consisting of
a tenth primer set including a primer comprising a nucleic acid of SEQ ID NO: 19 and a primer comprising a nucleic acid of SEQ ID NO: 20; andan eleventh primer set including a primer comprising a nucleic acid of SEQ ID NO: 21 and a primer comprising a nucleic acid of SEQ ID NO: 22; mixing a polymerase, an RNase, and an amplification buffer with the mixed sample to amplify the DNA; and detecting an increase of a signal emitted from a label on the probe; wherein an e13a2, e14a2, e13a3, or e14a3 breakpoint of the CML gene is detected.