Methods for determining a prognosis for survival for a patient with breast cancer

摘要

A method for determining a prognosis for survival for a patient with breast cancer is described. Also described is a method for monitoring the effectiveness of a course of treatment for a patient with breast cancer, and the use of such a method in a kit. A kit determining the level of RINF is also described.

主权项

1. A method for determining a prognosis for survival for a patient with breast cancer, comprising:
(a) measuring the level of CXXC5 expression in a biological sample from said patient, wherein said biological sample is a tumor, or a tumor biopsy or neoplastic cell containing sample, and wherein said measuring step is performed by
i) obtaining total RNA from the biological sample,ii) synthesizing first strand cDNA using the total RNA,iii) performing quantitative PCR of the first strand cDNA using
a) primers specific for amplifying first strand cDNA made from CXXC5mRNA, wherein the primers are 5′-tccgctgctctggagaag-3′(SEQ ID NO: 4) and 5′cacacgagcagtgacattgc-3′ (SEQ ID NO: 5) andb) a fluorescently labeled probe specific for detecting amplified first strand cDNA made from CXXC5 mRNA, wherein the fluorescently labeled probe is 6-FAM-aacccaaagctgccctctcc-BBQ (SEQ ID NO: 8), andiv) quantifying the level of CXXC5 mRNA expression in the biological sample by detecting fluorescence from the fluorescently labeled probe; (b) comparing the level of CXXC5 expression in said sample to a reference level of CXXC5, and (c) determining that said patient has an increased likelihood of survival if the level of CXXC5 expression is lower than the reference level or determining that said patient has a decreased likelihood of survival if the level of CXXC5 expression is higher than the reference level.