发明名称 |
TRANSFERING POLYNUCLEOTIDES INTO EUKARYOTIC CELLS USING CO-LIPOFECTION COMPLEXES OF A CATIONIC LIPID AND THE POLYNUCLEOTIDE |
摘要 |
The present invention provides methods and compositions for transferring large transgene polynucleotides and unlinked selectable marker polynucleotides into eukaryotic cells by a novel method designated co-lipofection. The methods and compositions of the invention are used to produce novel transgenic non-human animals harboring large transgenes, such as a transgene comprising a human APP gene or human immunoglobulin gene. |
申请公布号 |
NZ253943(A) |
申请公布日期 |
1997.01.29 |
申请号 |
NZ19930253943 |
申请日期 |
1993.06.18 |
申请人 |
GENPHARM INTERNATIONAL INC |
发明人 |
CHOI, THEODORE KYU;LORING, JEANNE FRANCES;KAY, ROBERT MAXWELL |
分类号 |
A01K67/027;C07K14/47;C07K16/00;C12N5/10;C12N15/09;C12N15/85;C12N15/88;(IPC1-7):C12N15/00;C12N15/12;C12N15/31;C12N5/00;A61K31/70;A61K35/12 |
主分类号 |
A01K67/027 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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