| 摘要 |
The present invention relates to a naturally occuring, isolated and characterized microorganism producing hydrolase enzyme, specifically, chitosanase; chitosanase isolated thereof; the nucleic acid sequence of the portion of the gene encoding this chitosanase; complete amino acid sequence of this chitosanase; hybrid plasmids containing the related gene; host microorganisms transformed with the plasmids; recombinant microorganisms overexpressing the enzyme; and to enzymatic treatment of chitosan with the aforementioned enzyme resulting in molecular weight decrease, viscosity decrease and increase of solubility of chitosan in water.
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