摘要 |
Novel chemiluminescent reagents undergoing chemiluminescence in the presence of peroxides depending on the amount of peroxidase and chemiluminescence analysis methods with the use of the same, for example, a method for assaying peroxidase activity by using chemiluminescence and a method for detecting and quantitating various substances by enzyme immunoassay with the use of peroxidase as a labeling. More particularly, the chemiluminescent reagents contain as the major components N,N'-disubstituted-9,9'-bisacridinium salt charge transfer complexes and N,N-disubstituted carboxamide compounds optionally together with specific amino alcohols. The methods comprise assaying peroxidase activity at a high sensitivity in the presence of peroxides with the use of these reagents. A chemiluminescent enzyme immunoassay method wherein a substance to be assayed can be assayed at an elevated sensitivity by effecting a chemiluminescent reaction with the use of the above reagents in the enzyme immunoassay method with the use of peroxidase as a labeling.
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申请人 |
DAINICHISEIKA COLOR & CHEMICALS MFG. CO., LTD.;SUZUKI, HIDEAKI;TAKAHASHI, KIYOSHI;ARAYA, GEN-ICHIRO;KATSURAGI, HISASHI;HOSOGOE, MIO |
发明人 |
SUZUKI, HIDEAKI;TAKAHASHI, KIYOSHI;ARAYA, GEN-ICHIRO;KATSURAGI, HISASHI;HOSOGOE, MIO |